Journal: Sensors (Basel, Switzerland)

Article Title: Use of a Multiplexed CMOS Microarray to Optimize and Compare Oligonucleotide Binding to DNA Probes Synthesized or Immobilized on Individual Electrodes

doi: 10.3390/s100807371

Figure Lengend Snippet: Fluorescence detection of rehybridization by target oligonucleotide to probes on the microarray used in following stripping at 95 °C for 1 h. Different concentrations (0, 2, 20, or 200 pM) of biotinylated target oligonucleotide were incubated in individual chambers of a four-chamber hyb cap, and binding was detected using Cy5-SA. ( A ) Complementary 5′-aminated DNA probe immobilized on Ppy deposited using constant current from 10 to 520 nA for 1.0 s. (B) Same as (4A), but a complementary DNA probe with an 5′-aminated T-linker was immobilized on the Ppy. ( C ) Same as (4A), but a non-complementary DNA probe with a 5′-aminated T-linker was immobilized on the Ppy.

Article Snippet: Data were quantified using Microarray Imager or ElectraSense software (CombiMatrix Corp.) for fluorescent scans or ECD respectively.

Techniques: Fluorescence, Microarray, Stripping Membranes, Incubation, Binding Assay

Journal: Sensors (Basel, Switzerland)

Article Title: Use of a Multiplexed CMOS Microarray to Optimize and Compare Oligonucleotide Binding to DNA Probes Synthesized or Immobilized on Individual Electrodes

doi: 10.3390/s100807371

Figure Lengend Snippet: Effects on ECD of adding a 5′-aminated 20 T-linker to DNA probes and preheating the immobilized probes prior to hybridization. Polypyrrole was deposited at 30 nA, and 20 pM of biotinylated oligonucleotide was hybridized on the array. A second microarray was incubated in 2XPBST for 1 h at 95 °C and washed once in PBS prior to hybridization.

Article Snippet: Data were quantified using Microarray Imager or ElectraSense software (CombiMatrix Corp.) for fluorescent scans or ECD respectively.

Techniques: Hybridization, Microarray, Incubation